"in vivo" electrophysiological recording in mouse

[diego]

Hi,
As this is my first comment, I will first congratulate the administrators for the idea, and hope that the forum goes on being a useful tool for students and young investigators.
 
In two weeks, I’m supposed to start with electrophysiological “in vivo” mouse recordings but I have not found much literature about that topic if anyone can give me some reference I will be very grateful. From other animals, like rats and cats is there enough information but from mouse seems complicated to find something. I’m also eager to know about other techniques where these animals are maintained anesthetized more than 3 hours.
Any help would be appreciated.

[Thi]

Hi,
could you further specify what kind of recording technique (i.e. patch-clamp, field potentials etc.) and which brain structure you will be looking at? There is actually a lot of literature on in vivo recordings in mice.

[diego]

Yes, I think you are true, I didn’t specify too much and there’s actually plenty of information about “in vivo” experiments with mice. So, I will try to specify a little bit:
We will attempt "whole-cell" (a patch-clamp modality) recordings in Thalamus. The point is how to maintain a mouse for as long as possible anesthetized, fixed and physiologically stable in a way that you can introduce a patch-clamp electrode in its brain , have successful seals and thus record neurons from the Thalamus.
I thought that maybe, some of you could have found a review or article about any of these topics that you find interesting or just make any suggestion or personal comment.
I already, found one very interesting paper "In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain" (Margrie et al. 2002).
Thank you for your interest and help.

[arnab]

I dunno if this helps but people in my lab do in vivo imaging of the mouse visual cortex and certainly keep the animal anesthetized for more than 3 hours. Look for publications from Mark Hübner from my lab for details.

Best
Arnab

[diego]

thanks,
i'll take a look at these papers.