Axon identification in biocytin labeling

[Thi]

Hey,

I was wondering whether any of you could give me advise how one can distinguish the axon from all the denrites in biocytin labeled (inter-)neurons.
I know there are general clues in terms of its smaller size, the myelin sheath that gives it a rather uneven surface and so forth, but yet it seems almost impossible to me.
Is there a good approach you can recommend?
Thanks!
Thi

[madaloc]

Stop.. let's discuss.. biocytin is useful in tracing axonal anterograde transport, therefore axons should be exhibited brighter than axons. To trace dendritic arbor, we use BDA, dont we? It exhibits dendritic arbor carefully. Although biocytin allows the researcher to find very thin axons, terminal ramifications, we seldom use it for tracing dendrites. It seemed to me this way )

try this: http://www.ncbi.nlm.nih.gov/pubmed/1384763?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_Discovery_RA&linkpos=4
and this: http://www.ncbi.nlm.nih.gov/pubmed/2479450?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_Discovery_RA&linkpos=1
but these articles are not for free (( are u agree with my arguments? 

[Thi]

Hum... I thought biocytin passively diffuses into the cell and binds to intracellular membrane components (which I can't remember...). However, I think I more or less solved my problem. If one knows the axonal projections, it is relatively easy to identify the axon using camera lucida reconstructions.